THE INTERACTION BETWEEN ACTOMYOSIN AND ADENOSINE TRIPHOSPHATE. LIGHT SCATTERING STUDIES
نویسندگان
چکیده
منابع مشابه
The Reaction between Actomyosin and Adenosine Triphosphate
1. A study is made of the effect of adenosine triphosphate (ATP) upon the viscosity of solutions of actomyosin in 0.5 M KCl. 2. The observed effects are discussed in terms of an initial drop of the viscosity (viscosity response) and its subsequent slow reversal (recovery effect). The latter is ascribed to a decrease in the ATP concentration through enzymatic hydrolysis. 3. The recovery effect i...
متن کاملMechanism of adenosine triphosphate hydrolysis by actomyosin.
The hydrolysis of ATP by acto-HMM has been studied during the transient state using a rapid-mixing apparatus. The rate of substrate binding was slightly slower and the rate of hydrolysis of the first molecule of ATP was essentially the same as for heavy meromyosin (HMM) alone. The rate of acto-HMM dissociation after binding substrate was too fast to measure in a stopped-flow apparatus, conseque...
متن کاملStudies on Adenosine Triphosphate Transphosphorylases
The isolation of the crystalline enzymes, adenosine triphosphate-creatine transphosphorylase (1) and adenosine triphosphate-adenosine 5’-phosphate transphosphorylase (2), made possible a comparative approach to the general problem of the mechanism of action of the adenosine triphosphate transphosphorylases. The present series of papers extends earlier studies from this laboratory on these enzym...
متن کاملInteraction of actomyosin with adenosine triphosphate at low ionic strength. II. Factors influencing clearing and superprecipitation: adenosine triphosphatase and birefringence of flow studies.
The clearing of actomyosin at p = 0.15 to 0.16 and pH 7.0 brought about by adenosine triphosphate in the presence of added magnesium was interpreted in the preceding paper in terms of the dissociation of actomyosin into its components (2). This report deals with various factors influencing the clearing reaction and the accompanying changes in adenosine triphosphatase activity. Adenosine triphos...
متن کاملAcyl adenylates; the interaction of adenosine triphosphate and L-methionine.
Several reactions are now known which can account for the exchange of PI” with the terminal pyrophosphate group of ATP (1-6). Recently it was demonstrated (3, 4) that the reaction of ATP and acetate with the acetate-activating enzyme (aceto-CoA-kinase) resulted in such an exchange. Because of this and other observations (4), it was proposed that the acetatedependent exchange occurred via the in...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1956
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)65316-5